論文

査読有り
2017年4月

Mesenchymal stem cells attenuate ischemia-reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study.

Surgery today
  • Tatsuaki Watanabe
  • ,
  • Yasushi Hoshikawa
  • ,
  • Naoya Ishibashi
  • ,
  • Hirotoshi Suzuki
  • ,
  • Hirotsugu Notsuda
  • ,
  • Yui Watanabe
  • ,
  • Masafumi Noda
  • ,
  • Masahiko Kanehira
  • ,
  • Shinya Ohkouchi
  • ,
  • Takashi Kondo
  • ,
  • Yoshinori Okada

47
4
開始ページ
425
終了ページ
431
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00595-016-1391-8
出版者・発行元
SPRINGER

PURPOSE: Mesenchymal stem cells (MSCs) suppress inflammation and immune responses. We conducted this study to find out if MSCs attenuate ischemia-reperfusion injury in a mouse model of lung transplantation. METHODS: C57BL/6J mouse lungs perfused with low-potassium dextran glucose solution were preserved at 4 °C for 18 h. Human MSCs were slowly injected into the left pulmonary artery of the lung grafts, and orthotopic left lung transplantation was then performed. The lung isografts were reperfused for 6 h, and bronchoalveolar lavage fluid (BALF) from the left lung graft was collected. We measured the protein concentration, cell count, and proinflammatory cytokine concentrations in the BALF. RESULTS: The protein concentration and cell count in the BALF were significantly lower in the MSC-administered grafts than in the PBS-administered controls. Concentrations of proinflammatory cytokines, including IL-1β, IL-17A, and TNF-α, in BALF tended to be lower in the MSC-administered grafts than in the controls, but the difference was not significant. CONCLUSION: The pre-transplant administration of MSCs via the pulmonary artery of the lung graft attenuated ischemia-reperfusion injury after prolonged cold ischemia in this mouse model of lung transplantation.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1007/s00595-016-1391-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27484066
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397582600003&DestApp=WOS_CPL

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