2008年10月
Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- ,
- 巻
- 74
- 号
- 20
- 開始ページ
- 6333
- 終了ページ
- 6337
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/AEM.02846-07
- 出版者・発行元
- AMER SOC MICROBIOLOGY
A beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalGlyNAcP) homolog gene was cloned from Vibrio vulnificus CMCP6. In synthetic reactions, the recombinant enzyme acted only with GlcNAc and GalNAc as acceptors in the presence of alpha-D-galactose-1-phosphate as a donor to form lacto-N-biose I (LNB) (Gal beta 1 -> 3GlcNAc) and galacto-N-biose (GNB) (Gal beta 1 -> 3GalNAc), respectively. GlcNAc was a much better acceptor than GalNAc. The enzyme also phosphorolysed LNB faster than it phosphorolysed GNB, and the k(cat)/K(m) for LNB was approximately 60 times higher than the k(cat)/K(m) for GNB. This result indicated that the enzyme was remarkably different from GalGlyNAcP from Bifidobacterium longum, which has similar activities with LNB and GNB, and GalGlyNAcP from Clostridium perfringens, which is a GNB-specific enzyme. The enzyme is the first LNB-specific enzyme that has been found and was designated lacto-N-biose I phosphorylase. The discovery of an LNB-specific GalGlyNAcP resulted in recategorization of bifidobacterial GalGlyNAcPs as galacto-N-biose/lacto-N-biose I phosphorylases.
- リンク情報
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- DOI
- https://doi.org/10.1128/AEM.02846-07
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18723650
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000259985300021&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=54949156444&origin=inward
- ID情報
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- DOI : 10.1128/AEM.02846-07
- ISSN : 0099-2240
- PubMed ID : 18723650
- SCOPUS ID : 54949156444
- Web of Science ID : WOS:000259985300021