2005年3月
Novel PCR-mediated mutagenesis employing DNA containing a natural abasic site as a template and translesional Taq DNA polymerase
JOURNAL OF BIOTECHNOLOGY
- ,
- ,
- 巻
- 116
- 号
- 3
- 開始ページ
- 227
- 終了ページ
- 232
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.biotec.2004.10.016
- 出版者・発行元
- ELSEVIER SCIENCE BV
We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR. (C) 2004 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.biotec.2004.10.016
- ISSN : 0168-1656
- PubMed ID : 15707683
- Web of Science ID : WOS:000227313600003