論文

査読有り 国際誌
2020年2月27日

The kinase PERK represses translation of the G protein-coupled receptor LGR5 and receptor tyrosine kinase ERBB3 during ER stress in cancer cells.

The Journal of biological chemistry
  • Yuka Okamoto
  • ,
  • Takuya Saito
  • ,
  • Yuri Tani
  • ,
  • Tamami Toki
  • ,
  • Akiko Hasebe
  • ,
  • Masaru Koido
  • ,
  • Akihiro Tomida

記述言語
英語
掲載種別
DOI
10.1074/jbc.RA119.010655

As a branch of the unfolded protein response, protein kinase R-like endoplasmic reticulum kinase (PERK) represses global translation in response to endoplasmic reticulum (ER) stress. This pathophysiological condition is associated with the tumor microenvironment in cancer. Previous findings in our lab have suggested that PERK selectively represses translation of some mRNAs, but this possibility awaits additional investigation. In the present study, we show that a stem cell marker protein, leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), is rapidly depleted in colon cancer cells during ER stress, an effect that depended on the PERK-mediated translational repression. Indeed, the PERK inhibition led to the accumulation of premature, underglycosylated forms of LGR5, which were produced only at low levels during proper PERK activation. Unlike the mature LGR5 form, which is constitutively degraded regardless of PERK activation, the underglycosylated LGR5 exhibited a prolonged half-life and accumulated inside the cells without being expressed on the cell surface. We also found that Erb-B2 receptor tyrosine kinase 3 (ERBB3) is subjected to a similar regulated depletion by PERK, whereas epidermal growth factor (EGFR), stress-inducible heat shock protein family A (Hsp70) member 5 (HSPA5), and anterior gradient 2 protein disulfide isomerase family member (AGR2) were relatively insensitive to the PERK-mediated repression of translation. These results indicate that LGR5 and ERBB3 are targets for PERK-mediated translational repression during ER stress.

リンク情報
DOI
https://doi.org/10.1074/jbc.RA119.010655
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32107308