Masayasu KUWAHARA - My portal

Profile Information

Affiliation: Professor, College of Humanities and Sciences Department of Chemistry, Nihon University

Degree: PhD(Mar, 1999, Okayama National University)

J-GLOBAL ID: 200901044806396635
researchmap Member ID: 1000359647

External link: https://eng.kuwaharaken.org/

High School Specialized Teacher’s Certificate (Science)1996/03
Junior High School Specialized Teacher’s Certificate (Science)1996/03
High School First-class Teacher's Certificate (Science)1994/03
Junior High School First-class Teacher's Certificate (Science)1994/03

Research Interests

Research Areas

Research History

Apr, 2018 - Present

Professor, Department of Chemistry, College of Humanities and Sciences, Nihon University
Apr, 2013 - Mar, 2018

Associate Professor, Graduate School of Science and Technology, Gunma University, National University Corporation
Dec, 2009 - Mar, 2013

Associate Professor, Graduate School of Engineering, Gunma University, National University Corporation
Apr, 2007 - Nov, 2009

Assistant Professor, Graduate School of Engineering, Gunma University, National University Corporation
Oct, 2004 - Mar, 2008

PRESTO Researcher (concurrent post), Strategic Basic Research Program (PRESTO), Japan Science and Technology Agency (JST)
Apr, 2004 - Mar, 2007

Research Associate, Department of Applied Chemistry, Faculty of Engineering, Gunma University, National University Corporation
Jun, 2001 - Mar, 2004

Research Associate, Department of Applied Chemistry, Faculty of Engineering, Gunma National University
Apr, 2000 - May, 2001

Research Associate, Department of Chemistry, University of Virginia (USA)
Apr, 1999 - Mar, 2000

Adjunct Lecturer, Faculty of Engineering, Okayama National University

Education

Awards

Apr, 2009

Minister of Education, Culture, Sports, Science and Technology Commendation Young Scientists' Prize

Major Papers

DNA polymerase variants with high processivity and accuracy for encoding and decoding locked nucleic acid sequences

Hidekazu Hoshino, Yuuya Kasahara, Masayasu Kuwahara, Satoshi Obika

Journal of the American Chemical Society, 142(51) 21530-21537, Dec 23, 2020 Corresponding author
Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan: In situ in the living body

Andrea Idili, Netzahualcóyotl Arroyo-Currás, Kyle L. Ploense, Andrew T. Csordas, Masayasu Kuwahara, Tod E. Kippin, Kevin W. Plaxco

Chemical Science, 10(35) 8164-8170, 2019
In situ condensation of an anti-cancer drug into fibrin gel enabling effective inhibition of tumor cell growth

Masayasu Kuwahara, Hiroto Fujita, Yuka Kataoka, Yasuyo Nakajima, Masanobu Yamada, Naoki Sugimoto

Chemical Communications, 55(78) 11679-11682, 2019 Lead authorCorresponding author
Novel one-tube-one-step real-time methodology for rapid transcriptomic biomarker detection: Signal amplification by ternary initiation complexes

Hiroto Fujita, Yuka Kataoka, Seiji Tobita, Masayasu Kuwahara, Naoki Sugimoto

Analytical Chemistry, 88(14) 7137-7144, Jul 19, 2016 Corresponding author
Minimal thioflavin T modifications improve visual discrimination of guanine-quadruplex topologies and alter compound-induced topological structures

Yuka Kataoka, Hiroto Fujita, Yuuya Kasahara, Toshitada Yoshihara, Seiji Tobita, Masayasu Kuwahara

Analytical Chemistry, 86(24) 12078-12084, Dec 16, 2014 Corresponding author
Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs

Debabrata Mandal, Caroline Köhrer, Dan Su, I. Ramesh Babu, Clement T.Y. Chan, Yuchen Liu, Dieter Söll, Paul Blum, Masayasu Kuwahara, Peter C. Dedon …

RNA, 20(2) 177-188, Feb, 2014
Efficacy of base-modification on target binding of small molecule DNA aptamers

Yuri Imaizumi, Yuuya Kasahara, Hiroto Fujita, Shunsuke Kitadume, Hiroaki Ozaki, Tamaki Endoh, Masayasu Kuwahara, Naoki Sugimoto

Journal of the American Chemical Society, 135(25) 9412-9419, Jun 26, 2013 Corresponding author
Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: Target binding dominated by 2'- O,4'-C-methylene-bridged/locked nucleic acid primer

Yuuya Kasahara, Yuuta Irisawa, Hiroto Fujita, Aiko Yahara, Hiroaki Ozaki, Satoshi Obika, Masayasu Kuwahara

Analytical Chemistry, 85(10) 4961-4967, May 21, 2013 Corresponding author
Artificially created nucleic acids and peptides/proteins in chemical biology

Masayasu Kuwahara, Yingfu Li, Eriks Rozners, Hiroshi Murakami

Journal of Nucleic Acids, 2013, 2013 Lead authorCorresponding author
Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides

Masayasu Kuwahara, Satoshi Obika, Jun Ichi Nagashima, Yuki Ohta, Yoshiyuki Suto, Hiroaki Ozaki, Hiroaki Sawai, Takeshi Imanishi

Nucleic Acids Research, 36(13) 4257-4265, Aug, 2008 Lead authorCorresponding author
Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity

Atsushi Shoji, Masayasu Kuwahara, Hiroaki Ozaki, Hiroaki Sawai

Journal of the American Chemical Society, 129(5) 1456-1464, Feb 7, 2007
Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides

Shu Ichi Nakano, Toshimasa Kirihata, Satoshi Fujii, Hiroshi Sakai, Masayasu Kuwahara, Hiroaki Sawai, Naoki Sugimoto

Nucleic Acids Research, 35(2) 486-494, Jan, 2007
Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

Masayasu Kuwahara, Jun Ichi Nagashima, Masatoshi Hasegawa, Takehiro Tamura, Rina Kitagata, Kazuo Hanawa, Shin Ichi Hososhima, Toshiyuki Kasamatsu, Hiroaki Ozaki, Hiroaki Sawai

Nucleic Acids Research, 34(19) 5383-5394, Nov, 2006 Lead authorCorresponding author
Nucleic acid with guanidinium modification exhibits efficient cellular uptake

Tatsuo Ohmichi, Masayasu Kuwahara, Naoko Sasaki, Masatoshi Hasegawa, Takahito Nishikata, Hiroaki Sawai, Naoki Sugimoto

Angewandte Chemie - International Edition, 44(41) 6682-6685, Oct 21, 2005
Design and optimization of camptothecin conjugates of triple helix-forming oligonucleotides for sequence-specific DNA cleavage by topoisomerase I

Paola B. Arimondo, Alexandre Boutorine, Brigitte Baldeyrou, Christian Bailly, Masayasu Kuwahara, Sidney M. Hecht, Jian Sheng Sun, Thérèse Garestier, Claude Hélène

Journal of Biological Chemistry, 277(5) 3132-3140, Feb 1, 2002
Hybridization between oxy-peptide nucleic acids and DNAs: Dependence of hybrid stabilities on the chain-lengths, types of base pairs, and the chain directions

Masayasu Kuwahara, Miki Arimitsu, Masanori Shigeyasu, Naho Saeki, Masahiko Sisido

Journal of the American Chemical Society, 123(20) 4653-4658, 2001 Lead author
Novel peptide nucleic acid that shows high sequence specificity and all- or-none-type hybridization with the complementary DNA

Masayasu Kuwahara, Miki Arimitsu, Masahiko Sisido

Journal of the American Chemical Society, 121(1) 256-257, Jan 13, 1999 Lead author

Major Books and Other Publications

Handbook of chemical biology of nucleic acids (edited by Naoki Sugimoto)

Fujita H, Kuwahara M (Role: Contributor, Detection systems using the ternary complex formation of nucleic acids)

Springer, Singapore, Oct 31, 2022 (ISBN: 9789811613135)
Modified nucleic acids in biology and medicine (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)

Kuwahara M, Hagiwara K, Ozaki H (Role: Contributor, Polymerase reactions that involve modified nucleotides)

Springer, Heidelberg, Aug 9, 2016 (ISBN: 9783319341736)
Chemical biology of nucleic acids: Fundamentals and clinical applications (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)

Kuwahara M (Role: Contributor, Progress in chemically modified nucleic acid aptamers)

Springer, Heidelberg, May 6, 2014 (ISBN: 9783642544514)
DNA sequencing–methods and apprications (edited by Anjana Munshi)

Kuwahara M, Sugimoto N (Role: Contributor, Nucleic acid aptamers as molecular tags for omics analyses involving sequencing)

INTECH-Open Access Publisher, Croatia, Apr 20, 2012 (ISBN: 9535105647)

Major Presentations

Enhanced selectivity over topology and altered targeting by fluorescent G4 binder modifications

Kuwahara M.

Asian 3 Roundtable on Nucleic Acids 2024 (A3RONA 2024), Nov 2, 2024, Nov 2, 2024 Invited
Fluorescent binders with enhanced selectivity for G-quadruplex topologies

Kuwahara M

Advances in Noncanonical Nucleic Acids (ANNA 2024), Oct 26, 2024, Oct 26, 2024 Invited
Halfway through the development of technology to create molecular recognition polymers that function in water

Kuwahara M

Supra FIBER International Summit for Nucleic Acids (S-FISNA), Feb 29, 2024 Invited
Modified substrates and polymerase variants for functional xeno nucleic acid selections

Kuwahara M

FIBER International Summit for Nucleic Acids 2023 (FISNA2023), Jul 19, 2023 Invited
Fluorescence molecular rotors Thioflavin T derivatives for various targets

Kuwahara M

The 2nd International Symposium on Functional Nucleic Acids (FNA2018), Nov 23, 2018 Invited
Development of unique nucleic acid materials for diagnosis and therapeutics

Kuwahara M

NanoTech Poland 2018, Jun 8, 2018 Invited
Xenonucleic acid aptamers: development towards in vitro selection of artificial biopolymer ligands/receptors

Kuwahara M

The 32nd International Symposium on Microscale Separations and Bioanalysis (MSB 2016), Apr 6, 2016 Invited
Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection using modified nucleic acid libraries

Kuwahara M

2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015), Dec 15, 2015 Invited
Functional nucleic acids with chemical modifications

Kuwahara M

POLYCHEM Seminar (Host: Prof. D.A.Schluter, Swiss Federal Institute of Technology Zurich (ETHZ)), Sep 14, 2011 Invited

Professional Memberships

Sep, 2017 - Present

Japan Society of Nucleic Acids Chemistry (JSNAC)
Nov, 2012 - Present

Japan Society for Analytical Chemistry (JSAC)
Aug, 2010 - Present

International Society for Nucleosides, Nucleotides and Nucleic Acids (IS3NA)
Apr, 2000 - Present

American Chemical Society (ACS)
Apr, 1999 - Present

Forum on Biomolecular Chemistry (FBC)
Apr, 1999 - Present

Society of Polymer Science, Japan (SPSJ)
Apr, 1999 - Present

Chemical Society of Japan (CSJ)

Major Industrial Property Rights

Major Media Coverage

What is the key to "opening up" for economic resumption? (TV Tokyo, World Business Satellite)

TV Tokyo, World Business Satellite, Jun 25, 2020 TV or radio program
Shionogi; to develop 30 minute corona diagnostic kit; contract with Nihon University etc. (Nihon Keizai Shimbun, Morning edition, First page)

Nihon Keizai Shimbun, Jun 22, 2020 Newspaper, magazine
Nihon University: Simple detection method for biomarkers, just put in reagents! (Nikkei Sangyo Shimbun, Morning edition, Page 6)

Nikkei Business Daily (Nikkei Inc.), Jan 14, 2020 Newspaper, magazine