2015年6月
RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
PLOS ONE
- 巻
- 10
- 号
- 6
- 開始ページ
- e0130397
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0130397
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
RSC (Remodel the Structure of Chromatin) is an ATP-dependent chromatin remodeling complex essential for the growth of Saccharomyces cerevisiae. RSC exists as two distinct isoforms that share core subunits including the ATPase subunit Nps1/Sth1 but contain either Rsc1 or Rsc2. Using the synthetic genetic array (SGA) of the non-essential null mutation method, we screened for mutations exhibiting synthetic growth defects in combination with the temperature-sensitive mutant, nps1-105, and found connections between mitochondrial function and RSC. rsc mutants, including rsc1 Delta, rsc2 Delta, and nps1-13, another temperature-sensitive nps1 mutant, exhibited defective respiratory growth; in addition, rsc2 Delta and nps1-13 contained aggregated mitochondria. The rsc2 Delta phenotypes were relieved by RSC1 overexpression, indicating that the isoforms play a redundant role in respiratory growth. Genome-wide expression analysis in nps1-13 under respiratory conditions suggested that RSC regulates the transcription of some target genes of the HAP complex, a transcriptional activator of respiratory gene expression. Nps1 physically interacted with Hap4, the transcriptional activator moiety of the HAP complex, and overexpression of HAP4 alleviated respiratory defects in nps1-13, suggesting that RSC plays pivotal roles in mitochondrial gene expression and shares a set of target genes with the HAP complex.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0130397
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26086550
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472808
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356567500102&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1371/journal.pone.0130397
- ISSN : 1932-6203
- PubMed ID : 26086550
- PubMed Central 記事ID : PMC4472808
- Web of Science ID : WOS:000356567500102