論文

査読有り 国際誌
2019年8月

Breeding of a cordycepin-resistant and adenosine kinase-deficient sake yeast strain that accumulates high levels of S-adenosylmethionine

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Muneyoshi Kanai
  • ,
  • Norito Yasuda
  • ,
  • Tomoko Morimoto
  • ,
  • Satoko Yoshida
  • ,
  • Nahoko Nishibori
  • ,
  • Masaki Mizunuma
  • ,
  • Tsutomu Fujii
  • ,
  • Haruyuki Iefuji

83
8
開始ページ
1530
終了ページ
1537
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2019.1571896
出版者・発行元
TAYLOR & FRANCIS LTD

Adenosine kinase (ADO1)-deficient mutants can be obtained from cordycepin-resistant strains, and the disruption of ADO1 causes S-adenosylmethionine (SAM) accumulation. To breed a high-SAM-accumulating yeast strain without genetic manipulation for industrial purposes, we bred a cordycepin-resistant strain using sake yeast kyokai No. 9 as the parent strain with a mutation in adenosine kinase (ADO1) and acquired high-SAM-accumulating strain. In the bred strain (NY9-10), a single mutation (T258I) was present in the ADO1, and this mutation site is an ATP binding site and is highly conserved during evolution. Moreover, it was suggested that high accumulation of SAM and cordycepin resistance in NY9-10 was due to functional deficiency of ADO1 by this mutation. This strain is not a genetically-modified organism and can be employed for use in the food and medicine industry such as mass production and sake making.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1080/09168451.2019.1571896
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30686113
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000475980300017&DestApp=WOS_CPL
URL
https://www.tandfonline.com/doi/full/10.1080/09168451.2019.1571896?utm_source=CPB&utm_medium=cms&utm_campaign=JOJ11420

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