論文

査読有り
2005年7月

Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells

JOURNAL OF BIOLOGICAL CHEMISTRY
  • M Maruyama
  • ,
  • T Ichisaka
  • ,
  • M Nakagawa
  • ,
  • S Yamanaka

280
26
開始ページ
24371
終了ページ
24379
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M501423200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Sox family transcription factors play essential roles in cell differentiation, development, and sex determination. Sox2 was previously thought to be the sole Sox protein expressed in mouse embryonic stem (ES) cells. Sox2 associates with Oct3/4 to maintain self- renewal of ES cells. In the current study, digital differential display identified transcripts for an additional Sox family member, Sox15, enriched in mouse ES cells. Reverse transcription-PCR confirmed that Sox15 expression is highest in undifferentiated ES cells and repressed upon differentiation. Sox15 is expressed at low levels in several tissues, including testis and muscle. In vitro studies showed that Sox15, like Sox2, associated with Oct3/4 on DNA sequences containing the octamer motif and Sox-binding site. Gel mobility shift assays and SELEX analyses showed that Sox15 binds similar DNA sequences as Sox2 but with weaker affinity. In contrast to the early embryonic lethality observed in Sox2-null mice, Sox15-null ES cells and mice were grossly normal. DNA microarray analyses revealed that Otx2, Ctgf, Ebaf, and Hrc are dysregulated in Sox15-null ES cells, however. Chromatin immunoprecipitation showed that Sox15, but not Sox2, bound to a Sox consensus binding site within the Hrc gene. Taken together, these data demonstrate differential roles for Sox15 and Sox2 in transcriptional control in mouse ES cells.

リンク情報
DOI
https://doi.org/10.1074/jbc.M501423200
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902294853118996
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15863505
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000230114000011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M501423200
  • ISSN : 0021-9258
  • J-Global ID : 200902294853118996
  • PubMed ID : 15863505
  • Web of Science ID : WOS:000230114000011

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