2012年5月
Human and Mouse Induced Pluripotent Stem Cells Are Differentially Reprogrammed in Response to Kinase Inhibitors
STEM CELLS AND DEVELOPMENT
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- 巻
- 21
- 号
- 8
- 開始ページ
- 1287
- 終了ページ
- 1298
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1089/scd.2011.0283
- 出版者・発行元
- MARY ANN LIEBERT INC
Conventional human induced pluripotent stem cells (hiPSCs), reprogrammed from somatic cells by induced expression of Oct4, Sox2, Klf4, and c-Myc, are phenotypically different from mouse embryonic stem cells (ESCs). In mice, culture in N2B27 serum-free 2i media (mitogen-activated protein kinase/extracellular signal-regulated kinase and glycogen synthase kinase 3 inhibitors; PD0325901 and CHIR99021) plus leukemia inhibitory factor (LIF) (2i + LIF medium) enriches for germline competent ESCs. Here, we demonstrate that flat-shaped hiPSC colonies can be reprogrammed into bowl-shaped multi-potent stem cells (2i-hiPSCs) by using 2i + LIF medium. Mechanical dissociation of 2i-hiPSC colonies enables stable maintenance for > 20 passages. Importantly, gene expression profiling demonstrated that 2i-hiPSCs more closely resemble primitive neural stem cells (PNSCs). Notably, this 2i-induced phenotype was generated from conventional hiPSCs, but not human ESCs (hESCs), thus correlating with the observation of neuroectodermal SOX1-positive colonies in conventional hiPSCs, but not hESCs in 2i + LIF medium. Thus, 2i-hiPSCs, which are nonteratoma forming PNSCs, may represent a safe source of cells for neural research and regenerative medicine.
- リンク情報
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- DOI
- https://doi.org/10.1089/scd.2011.0283
- CiNii Articles
- http://ci.nii.ac.jp/naid/120004247259
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21882976
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000303818900008&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1089/scd.2011.0283
- ISSN : 1547-3287
- CiNii Articles ID : 120004247259
- PubMed ID : 21882976
- Web of Science ID : WOS:000303818900008