論文

査読有り 筆頭著者 国際誌
2020年7月

UV irradiation of Type I collagen gels changed the morphology of the interconnected brain capillary endothelial cells on them.

Materials science & engineering. C, Materials for biological applications
  • Hideki Mori
  • ,
  • Masayuki Hara

112
開始ページ
110907
終了ページ
110907
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.msec.2020.110907

We cultured mouse brain capillary endothelial cell line bEnd.3 on the UV-irradiated Type I collagen gel. Morphology of bEnd.3 cells on the Type I collagen gel was drastically changed if the gel was crosslinked by UV irradiation. The interconnecting network of bEnd.3 cells which have cord-like morphology on the soft collagen gels was converted to the monolayer of the flat cells, tightly-bound each other covering the gel surface, in a confluent state. The collagen gels were mechanically stiffened by UV irradiation for 15 min with UV light at 254 nm showing approximately two times higher value of Young's modulus E (1.51 ± 0.58 kPa) than the control gel (3.17 ± 1.17 kPa). AFM images of the collagen fibrils were not severely changed after irradiation. Collagen subunit proteins were crosslinked and degraded simultaneously under UV irradiation proved by results of SDS-PAGE and separation by centrifugation. Expression of Integrin gene was measured by quantitative real-time PCR. Expression of the integrin α2 gene, tight junction protein 1 gene, and claudin 5 gene were down-regulated in cells on the UV irradiated collagen gel in comparison with the unirradiated one while expression of the integrin β1 gene and Integrin α1 gene did not significantly change. Thick actin filaments were more clearly observed in the cells on the UV-irradiated collagen gel than the unirradiated one by fluorescent microscopy. We conclude that UV irradiation made the collagen gel stiffened and changed the physiological state of bEnd.3 cells including their adhesion, extension, and proliferation.

リンク情報
DOI
https://doi.org/10.1016/j.msec.2020.110907
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32409061
ID情報
  • DOI : 10.1016/j.msec.2020.110907
  • PubMed ID : 32409061

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