2016年7月
Proplatelet formation in megakaryocytes is associated with endoplasmic reticulum stress.
Genes to cells : devoted to molecular & cellular mechanisms
- ,
- 巻
- 21
- 号
- 7
- 開始ページ
- 798
- 終了ページ
- 806
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/gtc.12384
Although previous studies suggest that proplatelet formation in megakaryocytes involves caspase-3, the mechanism underlying the activation of caspase-3 is unknown. Here, we analyzed caspase activation in a human megakaryoblastic cell line, MEG-01, which forms proplatelets spontaneously. Specific activation of caspase-3 and caspase-4 was found in proplatelets. Consistent with previous observations of caspase-4 autoactivation in response to endoplasmic reticulum (ER) stress, several ER stress marker proteins were expressed during proplatelet formation. A pharmacological ER stressor enhanced platelet production via proplatelet formation, whereas inhibition of caspase-4 caused suppression. These results suggest that ER stress is a mechanism underlying the maturation of megakaryocytes.
- ID情報
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- DOI : 10.1111/gtc.12384
- PubMed ID : 27296088