MISC

2005年2月

Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • J Futami
  • M Kitazoe
  • T Maeda
  • E Nukui
  • M Sakaguchi
  • J Kosaka
  • M Miyazaki
  • M Kosaka
  • H Tada
  • M Seno
  • Y Sasaki
  • NH Huh
  • M Namba
  • H Yamada
  • 全て表示

99
2
開始ページ
95
終了ページ
103
記述言語
英語
掲載種別
DOI
10.1263/jbb.99.095
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

In the post-genomic era, there is pressing need for development of protein manipulation methodology to analyze functions of proteins in living cells. For this purpose, techniques to deliver functional proteins into living cells are currently being evaluated as alternative approaches to the introduction of transcriptionally active DNA. Here, we describe a novel method for efficient protein transduction into living cells in which a protein is simply cationized with polyethylenimine (PEI) by limited chemical conjugation. PEI-cationized proteins appear to adhere to the cell surface by ionic charge interaction and then internalize into cells in a receptor- and transporter-independent fashion. Since PEI is an organic macromolecule with a high cationic-charge density, limited coupling with PEI results in endowment of sufficient cationic charge to proteins without causing serious decline in their fundamental functions. A number of PEI-cationized proteins, such as ribonuclease (RNase), green fluorescent protein (GFP) and immunoglobulin (IgG), efficiently entered cells and functioned in the cytosol. Our results suggest that protein cationization techniques using PEI will be useful for the development of protein transduction technology.

リンク情報
DOI
https://doi.org/10.1263/jbb.99.095
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000228407400002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1263/jbb.99.095
  • ISSN : 1389-1723
  • Web of Science ID : WOS:000228407400002

エクスポート
BibTeX RIS