Sep, 2017
Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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- Volume
- 1061
- Number
- First page
- 209
- Last page
- 219
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.jchromb.2017.07.022
- Publisher
- ELSEVIER SCIENCE BV
In accordance with our previous study that was carried out to identify novel anti-tumor ingredients, chromatographic separation in combination with an anti-tumor activity assay was used for analysis of Cordyceps militaris extract in this study. Various modes of chromatography including reversed-phase, cation-exchange and anion exchange were used to separate components of Cordyceps militaris, which showed various chemical properties. Anti-tumor activity of each fraction was assessed by a Hoechst staining-based apoptosis assay using malignant melanoma MeWo cells. By these repeated approaches through chromatographic segregation and cell biological assay, we finally succeeded in identifying the target substance from a certain fraction that included neutral hydrophilic components using a pre-column and post-column chlorine adduct ionization LC APCI MS method. The target substance was a mono-carbohydrate, xylitol, that induced apoptotic cell death in MeWo cells but not in normal human OUMS-24 fibroblasts. This is the first study showing that Cordyceps militaris extract contains a large amount of xylitol. Thus, our results will contribute greatly to uncovering the mysterious multifunctional herbal drug Cordyceps militaris as an anti-tumor agent.
- Link information
- ID information
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- DOI : 10.1016/j.jchromb.2017.07.022
- ISSN : 1570-0232
- eISSN : 1873-376X
- Pubmed ID : 28750234
- Web of Science ID : WOS:000411542000029