論文

査読有り 国際誌
2014年6月15日

GANP regulates the choice of DNA repair pathway by DNA-PKcs interaction in AID-dependent IgV region diversification.

Journal of immunology (Baltimore, Md. : 1950)
  • Mohammed Mansour Abbas Eid
  • ,
  • Kazuhiko Maeda
  • ,
  • Sarah Ameen Almofty
  • ,
  • Shailendra Kumar Singh
  • ,
  • Mayuko Shimoda
  • ,
  • Nobuo Sakaguchi

192
12
開始ページ
5529
終了ページ
39
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4049/jimmunol.1400021

RNA export factor germinal center-associated nuclear protein (GANP) interacts with activation-induced cytidine deaminase (AID) and shepherds it from the cytoplasm to the nucleus and toward the IgV region loci in B cells. In this study, we demonstrate a role for GANP in the repair of AID-initiated DNA damage in chicken DT40 B cells to generate IgV region diversity by gene conversion and somatic hypermutation. GANP plays a positive role in IgV region diversification of DT40 B cells in a nonhomologous end joining-proficient state. DNA-PKcs physically interacts with GANP, and this interaction is dissociated by dsDNA breaks induced by a topoisomerase II inhibitor, etoposide, or AID overexpression. GANP affects the choice of DNA repair mechanism in B cells toward homologous recombination rather than nonhomologous end joining repair. Thus, GANP presumably plays a critical role in protection of the rearranged IgV loci by favoring homologous recombination of the DNA breaks under accelerated AID recruitment.

リンク情報
DOI
https://doi.org/10.4049/jimmunol.1400021
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24808370
ID情報
  • DOI : 10.4049/jimmunol.1400021
  • PubMed ID : 24808370

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