How small numbers of inhibitory neurons functionally keep balance with large numbers of excitatory neurons in the brain by controlling each other is a fundamental question. Especially, this study quantitatively evaluated a topological mechanism of interaction networks in terms of controlling abilities of individual cortical neurons to other neurons. Combination of simultaneous electrical recording of ~1000 neurons and a quantitative evaluation method of neuronal interactions including excitatory-inhibitory categories, enabled us to evaluate the influence of individual neurons not only about firing rate but also about their relative positions in the networks and controllable ability of other neurons. Especially, the result showed that inhibitory neurons have more controlling ability than excitatory neurons, and such neurons were more often observed in deep layers. Because the limited number of neurons in terms controlling ability were much smaller than neurons based on centrality measure and, of course, more directly selected neurons based on their ability to control other neurons, the selection method of important neurons will help not only to produce realistic computational models but also will help to stimulate brain to effectively treat imbalanced disease states.