2015年
Construction of a High-Mannose-Type Glycan Library by a Renewed Top-Down Chemo-Enzymatic Approach
Chemistry - A European Journal,
- ,
- ,
- ,
- ,
- ,
- 巻
- 21
- 号
- 8
- 開始ページ
- 3224
- 終了ページ
- 3233
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/chem.201405781
- 出版者・発行元
- WILEY-V C H VERLAG GMBH
A comprehensive method for the construction of a high-mannose-type glycan library by systematic chemo-enzymatic trimming of a single Man9-based precursor was developed. It consists of the chemical synthesis of a non-natural tridecasaccharide precursor, the orthogonal demasking of the non-reducing ends, and trimming by glycosidases, which enabled a comprehensive synthesis of high-mannose-type glycans in their mono-or non-glucosylated forms. It employed glucose, isopropylidene, and N-acetylglucosamine groups for blocking the A-, B-, and C-arms, respectively. After systematic trimming of the precursor, thirty-seven high-mannose-type glycans were obtained. The power of the methodology was demonstrated by the enzymatic activity of human recombinant N-acetylglucosaminyltransferase-I toward M7-M3 glycans, clarifying the substrate specificity in the context of high-mannose-type glycans.
- リンク情報
-
- DOI
- https://doi.org/10.1002/chem.201405781
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000349664300014&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84922344788&partnerID=MN8TOARS
- ID情報
-
- DOI : 10.1002/chem.201405781
- ISSN : 0947-6539
- eISSN : 1521-3765
- ORCIDのPut Code : 71978738
- SCOPUS ID : 84922344788
- Web of Science ID : WOS:000349664300014