論文

査読有り
2014年5月

Meiotic recombination cold spots in chromosomal cohesion sites

GENES TO CELLS
  • Masaru Ito
  • ,
  • Kazuto Kugou
  • ,
  • Jeffrey A. Fawcett
  • ,
  • Sachiko Mura
  • ,
  • Sho Ikeda
  • ,
  • Hideki Innan
  • ,
  • Kunihiro Ohta

19
5
開始ページ
359
終了ページ
373
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/gtc.12138
出版者・発行元
WILEY

Meiotic chromosome architecture called axis-loop structures' and histone modifications have been shown to regulate the Spo11-dependent formation of DNA double-strand breaks (DSBs) that trigger meiotic recombination. Using genome-wide chromatin immunoprecipitation (ChIP) analyses followed by deep sequencing, we compared the genome-wide distribution of the axis protein Rec8 (the kleisin subunit of meiotic cohesin) with that of oligomeric DNA covalently bound to Spo11, indicative of DSB sites. The frequency of DSB sites is overall constant between Rec8 binding sites. However, DSB cold spots are observed in regions spanning +/- 0.8kb around Rec8 binding sites. The axis-associated cold spots are not due to the exclusion of Spo11 localization from the axis, because ChIP experiments showed that substantial Spo11 persists at Rec8 binding sites during DSB formation. Spo11 fused with Gal4 DNA binding domain (Gal4BD-Spo11) tethered in close proximity (0.8kb) to Rec8 binding sites hardly forms meiotic DSBs, in contrast with other regions. In addition, H3K4 trimethylation (H3K4me3) remarkably decreases at Rec8 binding sites. These results suggest that reduced histone H3K4me3 in combination with inactivation of Spo11 activity on the axis discourages DSB hot spot formation.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1111/gtc.12138
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24635992
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000334787600001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/gtc.12138
  • ISSN : 1356-9597
  • eISSN : 1365-2443
  • PubMed ID : 24635992
  • Web of Science ID : WOS:000334787600001

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