論文

査読有り
2012年9月

A Central Coupler for Recombination Initiation Linking Chromosome Architecture to S Phase Checkpoint

MOLECULAR CELL
  • Tomoichiro Miyoshi
  • ,
  • Masaru Ito
  • ,
  • Kazuto Kugou
  • ,
  • Shintaro Yamada
  • ,
  • Masaki Furuichi
  • ,
  • Arisa Oda
  • ,
  • Takatomi Yamada
  • ,
  • Kouji Hirota
  • ,
  • Hisao Masai
  • ,
  • Kunihiro Ohta

47
5
開始ページ
722
終了ページ
733
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.molcel.2012.06.023
出版者・発行元
CELL PRESS

Higher-order chromosome structure is assumed to control various DNA-templated reactions in eukaryotes. Meiotic chromosomes implement developed structures called "axes" and "loops"; both are suggested to tether each other, activating Spoil to catalyze meiotic DNA double-strand breaks (DSBs) at recombination hotspots. We found that the Schizosaccharomyces pombe Spo11 homolog Red 12 and its partners form two distinct subcomplexes, DSBC (Rec6-Rec12-Rec14) and SFT (Rec7-Rec15-Rec24). Mde2, whose expression is strictly regulated by the replication checkpoint, interacts with Rec15 to stabilize the SFT subcomplex and further binds Rec14 in DSBC. Rec10 provides a docking platform for SFT binding to axes and can partially interact with DSB sites located in loops depending upon Mde2, which is indicative of the formation of multiprotein-based tethered axis-loop complex. These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint.

Web of Science ® 被引用回数 : 59

リンク情報
DOI
https://doi.org/10.1016/j.molcel.2012.06.023
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22841486
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000308972200007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.molcel.2012.06.023
  • ISSN : 1097-2765
  • PubMed ID : 22841486
  • Web of Science ID : WOS:000308972200007

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