論文

査読有り
2009年7月

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

MOLECULAR BIOLOGY OF THE CELL
  • Kazuto Kugou
  • ,
  • Tomoyuki Fukuda
  • ,
  • Shintaro Yamada
  • ,
  • Masaru Ito
  • ,
  • Hiroyuki Sasanuma
  • ,
  • Saori Mori
  • ,
  • Yuki Katou
  • ,
  • Takehiko Itoh
  • ,
  • Kouji Matsumoto
  • ,
  • Takehiko Shibata
  • ,
  • Katsuhiko Shirahige
  • ,
  • Kunihiro Ohta

20
13
開始ページ
3064
終了ページ
3076
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E08-12-1223
出版者・発行元
AMER SOC CELL BIOLOGY

Spo11-mediated DNA double-strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled. The meiotic cohesin Rec8 has been implicated in regulating DSB formation, but little is known about the features of their interplay. To elucidate this point, we investigated the genome-wide localization of Spo11 in budding yeast during early meiosis by chromatin immunoprecipitation using high-density tiling arrays. We found that Spo11 is dynamically localized to meiotic chromosomes. Spo11 initially accumulated around centromeres and thereafter localized to arm regions as premeiotic S phase proceeded. During this stage, a substantial proportion of Spo11 bound to Rec8 binding sites. Eventually, some of Spo11 further bound to both DSB and Rec8 sites. We also showed that such a change in a distribution of Spo11 is affected by hydroxyurea treatment. Interestingly, deletion of REC8 influences the localization of Spo11 to centromeres and in some of the intervals of the chromosomal arms. Thus, we observed a lack of DSB formation in a region-specific manner. These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation.

Web of Science ® 被引用回数 : 72

リンク情報
DOI
https://doi.org/10.1091/mbc.E08-12-1223
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19439448
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000267536400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1091/mbc.E08-12-1223
  • ISSN : 1059-1524
  • PubMed ID : 19439448
  • Web of Science ID : WOS:000267536400007

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