論文

査読有り
2016年6月

Arabidopsis AtRRP44 has ribonuclease activity that is required to complement the growth defect of yeast rrp44 Mutant

PLANT BIOTECHNOLOGY
  • Naoyoshi Kumakura
  • ,
  • Hiroka Otsuki
  • ,
  • Masaru Ito
  • ,
  • Mika Nomoto
  • ,
  • Yasuomi Tada
  • ,
  • Kunihiro Ohta
  • ,
  • Yuichiro Watanabe

33
2
開始ページ
77
終了ページ
+
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.5511/plantbiotechnology.16.0316a
出版者・発行元
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY

The RNA exosome is a multiprotein complex responsible for 3' to 5' degradation and processing of various classes of RNAs in eukaryotes. Rrp44/Dis3 is the catalytic center of the RNA exosome in yeast and human. Previously, we identified Arabidopsis thaliana RRP44 (AtRRP44) as a single functional homolog of Rrp44/Dis3. Although AtRRP44 is potentially a catalytic center of the plant RNA exosome, the ribonuclease activity of AtRRP44 has not been tested. Here, we show that AtRRP44 has ribonuclease activity using in vitro translated recombinant proteins. Mutation of the aspartic acid residue D489 of AtRRP44 to asparagine (D489N) resulted in loss of ribonuclease activity, indicating that aspartic acid is at the active site. The wild-type AtRRP44 protein rescued the growth defect of Saccharomyces cerevisiae rrp44 mutants, but the D489N mutated AtRRP44 did not. This finding suggests that the ribonuclease activity of wild-type AtRRP44 is required for yeast cell viability. We also showed that AtRRP44 was highly expressed in organs experiencing active cell turnover, such as shoot apical meristem, root apical meristem, and lateral root primordium. Along with previous studies showing that loss of RRP44 in Arabidopsis is lethal, our results suggest that AtRRP44 has ribonuclease activity that is related to plant development.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.5511/plantbiotechnology.16.0316a
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000379958400003&DestApp=WOS_CPL
ID情報
  • DOI : 10.5511/plantbiotechnology.16.0316a
  • ISSN : 1342-4580
  • Web of Science ID : WOS:000379958400003

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