論文

国際誌
2021年

Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes.

Methods in molecular biology (Clifton, N.J.)
  • Yan Yun
  • ,
  • Masaru Ito
  • ,
  • Sumit Sandhu
  • ,
  • Neil Hunter

2153
開始ページ
267
終了ページ
286
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-1-0716-0644-5_19

Crossing-over between homologous chromosomes is essential for accurate chromosome segregation at anaphase-I of meiosis. Defective crossing-over is associated with infertility, pregnancy miscarriage, and congenital disease. This chapter presents optimized protocols for the analysis of meiotic crossovers at the cytological level in spermatocytes and oocytes from mouse. The first approach employs immunocytology to detect MLH1, a DNA mismatch-repair protein that specifically marks crossover sites in the pachytene stage of meiotic prophase-I. These immunocytological methods have general utility for the analysis of other recombination steps, such as initiation and DNA strand exchange. The second approach visualizes chiasmata, the points of physical exchange between homologous chromosomes that are present during the diakinesis and metaphase-I stages. Both approaches are readily adaptable to the analysis of crossing over in other vertebrate species.

リンク情報
DOI
https://doi.org/10.1007/978-1-0716-0644-5_19
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32840786
ID情報
  • DOI : 10.1007/978-1-0716-0644-5_19
  • PubMed ID : 32840786

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