論文

査読有り
2017年7月26日

Analysis of environmental bacteria at single-cell level

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
  • Masahito Hosokawa
  • ,
  • Yohei Nishikawa
  • ,
  • Masato Kogawa
  • ,
  • Haruko Takeyama

開始ページ
634
終了ページ
637
記述言語
英語
掲載種別
研究論文(国際会議プロシーディングス)
DOI
10.1109/TRANSDUCERS.2017.7994129
出版者・発行元
Institute of Electrical and Electronics Engineers Inc.

Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.

リンク情報
DOI
https://doi.org/10.1109/TRANSDUCERS.2017.7994129
ID情報
  • DOI : 10.1109/TRANSDUCERS.2017.7994129
  • SCOPUS ID : 85029384193

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