論文

2020年3月6日

Massively parallel single-cell genome sequencing enables high-resolution analysis of soil and marine microbiome

  • Yohei Nishikawa
  • ,
  • Masato Kogawa
  • ,
  • Masahito Hosokawa
  • ,
  • Katsuhiko Mineta
  • ,
  • Kai Takahashi
  • ,
  • Chikako Sakanashi
  • ,
  • Hayedeh Behzad
  • ,
  • Takashi Gojobori
  • ,
  • Haruko Takeyama

DOI
10.1101/2020.03.05.962001
出版者・発行元
Cold Spring Harbor Laboratory

<title>Abstract</title>To improve our understanding of the environmental microbiome, we developed a single-cell genome sequencing platform, named SAG-gel, which utilizes gel beads for single-cell isolation, cell lysis, and whole genome amplification (WGA) for sequencing. SAG-gel enables serial, parallel and independent reactions of &gt; 100,000 single cells in a single tube, delivering high-quality genome recovery with storable randomized single-cell genome libraries. From soil and marine environmental sources, we acquired 734 partial genomes that are recapitulated in 231 species, 35% of which were assigned as high-to-medium qualities. We found that each genome to be almost unique and 98.7% of them were newly identified, implying the complex genetic diversities across 44 phyla. The various metabolic capabilities including virulence factors and biosynthetic gene clusters were found across the lineages at single-cell resolution. This technology will accelerate the accumulation of reference genomes of uncharacterized environmental microbes and provide us new insights for their roles.

リンク情報
DOI
https://doi.org/10.1101/2020.03.05.962001
URL
https://syndication.highwire.org/content/doi/10.1101/2020.03.05.962001
ID情報
  • DOI : 10.1101/2020.03.05.962001

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