2012年
Translational Homeostasis via the mRNA Cap-Binding Protein, eIF4E
Molecular Cell
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- 巻
- 46
- 号
- 6
- 開始ページ
- 847
- 終了ページ
- 858
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.molcel.2012.04.004
- 出版者・発行元
- CELL PRESS
Translational control of gene expression plays a key role in many biological processes. Consequently, the activity of the translation apparatus is under tight homeostatic control. elF4E, the mRNA 5' cap-binding protein, facilitates cap-dependent translation and is a major target for translational control. elF4E activity is controlled by a family of repressor proteins, termed 4E-binding proteins (4E-BPs). Here, we describe the surprising finding that despite the importance of elF4E for translation, a drastic knockdown of elF4E caused only minor reduction in translation. This conundrum can be explained by the finding that 4E-BP1 is degraded in elF4E-knock-down cells. Hypophosphorylated 4E-BP1, which binds to elF4E, is degraded, whereas hyperphosphorylated 4E-BP1 is refractory to degradation. We identified the KLHL25-CUL3 complex as the E3 ubiquitin ligase, which targets hypophosphorylated 4E-BP1. Thus, the activity of elF4E is under homeostatic control via the regulation of the levels of its repressor protein 4E-BP1 through ubiquitination.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.molcel.2012.04.004
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306097000014&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84862979524&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-5553-0311
- ID情報
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- DOI : 10.1016/j.molcel.2012.04.004
- ISSN : 1097-2765
- ORCIDのPut Code : 51064636
- SCOPUS ID : 84862979524
- Web of Science ID : WOS:000306097000014