2004年1月
Kinesin walks hand-over-hand
SCIENCE
- ,
- ,
- ,
- 巻
- 303
- 号
- 5658
- 開始ページ
- 676
- 終了ページ
- 678
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1126/science.1093753
- 出版者・発行元
- AMER ASSOC ADVANCEMENT SCIENCE
Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 +/- 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps.
- リンク情報
- ID情報
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- DOI : 10.1126/science.1093753
- ISSN : 0036-8075
- PubMed ID : 14684828
- Web of Science ID : WOS:000188530800042