<p>The accurate evaluation of story-collapsed buildings is important for human casualty estimation in seismic risk assessment and disaster response. This study investigates the story-collapsed buildings in Mashiki Town, that are identified based on damage survey data of the local government, due to the 2016 Kumamoto, Japan, earthquake. As the result of analysis from the viewpoints of structural material and construction period, the story-collapse ratio of wooden buildings was found to be much higher than that of other non-wooden buildings, and it becomes larger as the construction period gets older. By comparing this result with the estimated distributions of the peak ground velocity (PGV) and the Instrumental JMA seismic intensity, the fragility curves for story-collapsed buildings were developed. The developed fragility curves for Mashiki Town showed lower story-collapse ratios than those developed for Nishinomiya City in the 1995 Kobe Earthquake.</p>