In this study, we conducted building damage analysis using the damage survey data by the Uki City government, Kumamoto Prefecture due to the 2016 Kumamoto Earthquake, and constructed building fragility curves in combination with the estimated earthquake motion distribution. As the result of analysis on the tendency of damage ratios with respect to the structural material, construction period, roof types, and number of floors for wooden buildings, it was found that the major damage ratio of wooden buildings was larger than that of non-wooden buildings. The major damage ratio of wooden buildings in Uki City was significantly smaller than that of Mashiki Town for the same construction period and the same seismic motion level. The use of the both data from Uki City and Mashiki Town is suggested to establish unified fragility curves to cover a wide range of seismic motion.