2015年1月
Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation
CHEMBIOCHEM
- 巻
- 16
- 号
- 1
- 開始ページ
- 167
- 終了ページ
- 176
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/cbic.201402346
- 出版者・発行元
- WILEY-V C H VERLAG GMBH
We developed fluorescent turn-on probes containing a fluorescent nucleoside, 5-(benzofuran-2-yl) deoxyuridine (dU(BF)) or 5-(3-methylbenzofuran-2-yl) deoxyuridine (dU(MBF)), for the detection of single-stranded DNA or RNA by utilizing DNA triplex formation. Fluorescence measurements revealed that the probe containing dUMBF achieved superior fluorescence enhancement than that containing dU(BF). NMR and fluorescence analyses indicated that the fluorescence intensity increased upon triplex formation partly as a consequence of a conformational change at the bond between the 3-methylbenzofuran and uracil rings. In addition, it is suggested that the microenvironment around the 3-methylbenzofuran ring contributed to the fluorescence enhancement. Further, we developed a method for detecting RNA by rolling circular amplification in combination with triplex-induced fluorescence enhancement of the oligonucleotide probe containing dU(MBF).
- リンク情報
- ID情報
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- DOI : 10.1002/cbic.201402346
- ISSN : 1439-4227
- eISSN : 1439-7633
- Web of Science ID : WOS:000346781600021