論文

査読有り
2015年1月

Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation

CHEMBIOCHEM
  • Takashi Kanamori
  • Hiroki Ohzeki
  • Yoshiaki Masaki
  • Akihiro Ohkubo
  • Mari Takahashi
  • Kengo Tsuda
  • Takuhiro Ito
  • Mikako Shirouzu
  • Kanako Kuwasako
  • Yutaka Muto
  • Mitsuo Sekine
  • Kohji Seio
  • 全て表示

16
1
開始ページ
167
終了ページ
176
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cbic.201402346
出版者・発行元
WILEY-V C H VERLAG GMBH

We developed fluorescent turn-on probes containing a fluorescent nucleoside, 5-(benzofuran-2-yl) deoxyuridine (dU(BF)) or 5-(3-methylbenzofuran-2-yl) deoxyuridine (dU(MBF)), for the detection of single-stranded DNA or RNA by utilizing DNA triplex formation. Fluorescence measurements revealed that the probe containing dUMBF achieved superior fluorescence enhancement than that containing dU(BF). NMR and fluorescence analyses indicated that the fluorescence intensity increased upon triplex formation partly as a consequence of a conformational change at the bond between the 3-methylbenzofuran and uracil rings. In addition, it is suggested that the microenvironment around the 3-methylbenzofuran ring contributed to the fluorescence enhancement. Further, we developed a method for detecting RNA by rolling circular amplification in combination with triplex-induced fluorescence enhancement of the oligonucleotide probe containing dU(MBF).

リンク情報
DOI
https://doi.org/10.1002/cbic.201402346
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000346781600021&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cbic.201402346
  • ISSN : 1439-4227
  • eISSN : 1439-7633
  • Web of Science ID : WOS:000346781600021

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