論文

査読有り
2015年3月

Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells

SCIENTIFIC REPORTS
  • Setsuko Fujii
  • ,
  • Satomi Nishikawa-Torikai
  • ,
  • Yoko Futatsugi
  • ,
  • Yayoi Toyooka
  • ,
  • Mariko Yamane
  • ,
  • Satoshi Ohtsuka
  • ,
  • Hitoshi Niwa

5
開始ページ
9146
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep09146
出版者・発行元
NATURE PUBLISHING GROUP

Nuclear receptor subfamily 0, group B, member 1 (Nr0b1, also known as Dax1) is regarded as an important component of the transcription factor network that governs pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Nr0b1 using the Cre-loxP system and analyzed its precise function. We succeeded in establishing the Nr0b1-null ES cells and confirmed their pluripotency by showing their contribution to chimeric embryos. However, they proliferated slowly with over-expression of 2-cell stage specific transcripts including Zscan4c, which is known to be involved in telomere elongation in ES cells. We revealed that over-expression of Zscan4c prevents normal self-renewal by inducing arrest at G2 phase followed by cell death and that Nr0b1 directly represses the Zscan4c promoter. These data indicated that Nr0b1 is not essential to maintain pluripotency but is involved in the proper activation of 2-cell specific transcripts for self-renewal.

Web of Science ® 被引用回数 : 27

リンク情報
DOI
https://doi.org/10.1038/srep09146
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25772165
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351181400001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep09146
  • ISSN : 2045-2322
  • PubMed ID : 25772165
  • Web of Science ID : WOS:000351181400001

エクスポート
BibTeX RIS