論文

査読有り
2013年10月15日

Maintenance of pluripotency in mouse ES cells without Trp53

Scientific Reports
  • Masaki Shigeta
  • ,
  • Satoshi Ohtsuka
  • ,
  • Satomi Nishikawa-Torikai
  • ,
  • Mariko Yamane
  • ,
  • Setsuko Fujii
  • ,
  • Kazuhiro Murakami
  • ,
  • Hitoshi Niwa

3
開始ページ
2944
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep02944
出版者・発行元
Nature Publishing Group

Tumor suppressor Trp53 works as a guardian of the genome in somatic cells. In mouse embryonic stem (ES) cells, it was reported that Trp53 represses pluripotency-associated transcription factor Nanog to induce differentiation. However, since Trp53-null mice develop to term, Trp53 is dispensable for both the maintenance and differentiation of the pluripotent stem cell population in vivo, suggesting the differential functions of Trp53 in ES cells and embryos. To reveal the basis of this discrepancy, here we established a new line of Trp53-null ES cells by sequential gene targeting and evaluated their ability to differentiate in vitro and in vivo. We found that Trp53-null ES cells had defects in differentiation in vitro as reported previously, whereas they were able to contribute to normal development in chimeric embryos. These data indicated that the requirement of Trp53 for maintaining and executing the ES pluripotency is not absolute.

リンク情報
DOI
https://doi.org/10.1038/srep02944
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24126347
ID情報
  • DOI : 10.1038/srep02944
  • ISSN : 2045-2322
  • PubMed ID : 24126347
  • SCOPUS ID : 84903681954

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