論文

査読有り
2013年

In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

BIOMED RESEARCH INTERNATIONAL
  • Tomoko Nakanishi
  • ,
  • Rumiko Saito
  • ,
  • Makoto Taniguchi
  • ,
  • Haruka Oda
  • ,
  • Atsumi Soma
  • ,
  • Mayu Yasunaga
  • ,
  • Mariko Yamane
  • ,
  • Kenzo Sato

2013
開始ページ
895706
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1155/2013/895706
出版者・発行元
HINDAWI PUBLISHING CORPORATION

Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation. We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence. Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by in vivo bioluminescence. As the result, strong activity for ossification was observed with 1 alpha,25-hydroxyvitamin D3. Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1155/2013/895706
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23607098
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000317225000001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1155/2013/895706
  • ISSN : 2314-6133
  • PubMed ID : 23607098
  • Web of Science ID : WOS:000317225000001

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