論文

国際誌
2020年11月14日

Molecular detection of maturation stages in the developing kidney.

Developmental biology
  • Hidekazu Naganuma
  • ,
  • Koichiro Miike
  • ,
  • Tomoko Ohmori
  • ,
  • Shunsuke Tanigawa
  • ,
  • Takumi Ichikawa
  • ,
  • Mariko Yamane
  • ,
  • Masatoshi Eto
  • ,
  • Hitoshi Niwa
  • ,
  • Akio Kobayashi
  • ,
  • Ryuichi Nishinakamura

470
開始ページ
62
終了ページ
73
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ydbio.2020.11.002

Recent advances in stem cell biology have enabled the generation of kidney organoids in vitro, and further maturation of these organoids is observed after experimental transplantation. However, the current organoids remain immature and their precise maturation stages are difficult to determine because of limited information on developmental stage-dependent gene expressions in the kidney in vivo. To establish relevant molecular coordinates, we performed single-cell RNA sequencing (scRNA-seq) on developing kidneys at different stages in the mouse. By selecting genes that exhibited upregulation at birth compared with embryonic day 15.5 as well as cell lineage-specific expression, we generated gene lists correlated with developmental stages in individual cell lineages. Application of these lists to transplanted embryonic kidneys revealed that most cell types, other than the collecting ducts, exhibited similar maturation to kidneys at the neonatal stage in vivo, revealing non-synchronous maturation across the cell lineages. Thus, our scRNA-seq data can serve as useful molecular coordinates to assess the maturation of developing kidneys and eventually of kidney organoids.

リンク情報
DOI
https://doi.org/10.1016/j.ydbio.2020.11.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33197428
ID情報
  • DOI : 10.1016/j.ydbio.2020.11.002
  • PubMed ID : 33197428

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