This paper deals with an application of the predictive functional control with a state estimator-based internal model (PFC_EBIM). The PFC_EBIM has been shown to be effective in simulation. However, neither detailed experimental validation nor comparison with other controllers has been reported thus far. Here, the PFC_EBIM is implemented in a single-axis positioning system, and a few experimental tests are conducted. Tracking performance of the PFC_EBIM, standard PFC, and P-PI control for both smooth and non-smooth reference signals are compared. The experimental results prove the effectiveness of the PFC_EBIM.