論文

国際誌
2012年

Visualization of cortical projection neurons with retrograde TET-off lentiviral vector.

PloS one
  • Akiya Watakabe
  • ,
  • Shigeki Kato
  • ,
  • Kazuto Kobayashi
  • ,
  • Masafumi Takaji
  • ,
  • Yuki Nakagami
  • ,
  • Osamu Sadakane
  • ,
  • Masanari Ohtsuka
  • ,
  • Hiroyuki Hioki
  • ,
  • Takeshi Kaneko
  • ,
  • Hiroyuki Okuno
  • ,
  • Takashi Kawashima
  • ,
  • Haruhiko Bito
  • ,
  • Yoshihiro Kitamura
  • ,
  • Tetsuo Yamamori

7
10
開始ページ
e46157
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0046157

We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0046157
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23071541
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465318
ID情報
  • DOI : 10.1371/journal.pone.0046157
  • PubMed ID : 23071541
  • PubMed Central 記事ID : PMC3465318

エクスポート
BibTeX RIS