論文

査読有り
2020年12月22日

Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells.

The Journal of reproduction and development
  • Kotaro Horiguchi
  • ,
  • Saishu Yoshida
  • ,
  • Takehiro Tsukada
  • ,
  • Takashi Nakakura
  • ,
  • Ken Fujiwara
  • ,
  • Rumi Hasegawa
  • ,
  • Shu Takigami
  • ,
  • Shunji Ohsako

66
6
開始ページ
515
終了ページ
522
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1262/jrd.2020-082

Cluster of differentiation (CD) 9 and CD81 are closely-related members of the tetraspanin family that consist of four-transmembrane domain proteins. Cd9 and Cd81 are highly expressed in breast cancer cells; however, their expression in healthy mammary glands is unclear. In this study, we performed quantitative real-time PCR to analyze the expression levels of Cd9 and Cd81. Histological techniques were employed to identify Cd9- and Cd81-expressing cells in rat mammary glands during pregnancy and lactation. It was observed that Cd9 and Cd81 were expressed in the mammary glands, and their expression levels correlated with mammary gland development. To identify cells expressing Cd9 and Cd81 in the mammary glands, we performed double immunohistochemical staining for CD9 and CD81, prolactin receptor long form, estrogen receptor alpha, or Ki67. The results showed that CD9 and CD81 were co-expressed in proliferating mammary epithelial cells. Next, we attempted to isolate CD9-positive epithelial cells from the mammary gland using pluriBead cell-separation technology based on antibody-mediated binding of cells to beads of different sizes, followed by isolation using sieves with different mesh sizes. We successfully isolated CD9-positive epithelial cells with 96.8% purity. In addition, we observed that small-interfering RNAs against Cd9 and Cd81 inhibited estrogen-induced proliferation of CD9-positive mammary epithelial cells. Our current findings may provide novel insights into the proliferation of mammary epithelial cells during pregnancy and lactation as well as in pathological processes associated with breast cancer.

リンク情報
DOI
https://doi.org/10.1262/jrd.2020-082
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32830152
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768173
ID情報
  • DOI : 10.1262/jrd.2020-082
  • PubMed ID : 32830152
  • PubMed Central 記事ID : PMC7768173

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