論文

査読有り 筆頭著者 責任著者 国際誌
2021年2月

Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated capillary.

Cell and tissue research
  • Takashi Nakakura
  • ,
  • Takeshi Suzuki
  • ,
  • Hideyuki Tanaka
  • ,
  • Kenjiro Arisawa
  • ,
  • Toshio Miyashita
  • ,
  • Yoko Nekooki-Machida
  • ,
  • Toshiki Kurosawa
  • ,
  • Yuma Tega
  • ,
  • Yoshiharu Deguchi
  • ,
  • Haruo Hagiwara

383
2
開始ページ
823
終了ページ
833
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00441-020-03273-y

Endothelial fenestrae are transcellular pores that pierce the capillary walls in endocrine glands such as the pituitary. The fenestrae are covered with a thin fibrous diaphragm consisting of the plasmalemma vesicle-associated protein (PLVAP) that clusters to form sieve plates. The basal surface of the vascular wall is lined by basement membrane (BM) composed of various extracellular matrices (ECMs). However, the relationship between the ECMs and the endothelial fenestrae is still unknown. In this study, we isolated fenestrated endothelial cells from the anterior lobe of the rat pituitary, using a dynabeads-labeled antibody against platelet endothelial cell adhesion molecule 1 (PECAM1). We then analyzed the gene expression levels of several endothelial marker genes and genes for integrin α subunits, which function as the receptors for ECMs, by real-time polymerase chain reaction (PCR). The results showed that the genes for the integrin α subunit, which binds to collagen IV, fibronectin, laminin-411, or laminin-511, were highly expressed. When the PECAM1-positive cells were cultured for 7 days on collagen IV-, fibronectin-, laminins-411-, or laminins-511-coated coverslips, the sieve plate structures equipped with probably functional fenestrae were maintained only when the cells were cultured on fibronectin. Additionally, real-time PCR analysis showed that the fibronectin coating was effective in maintaining the expression pattern of several endothelial marker genes that were preferentially expressed in the endothelial cells of the fenestrated capillaries. These results indicate that fibronectin functions as the principal factor in the maintenance of the sieve plate structures in the endothelial cells of the fenestrated capillary.

リンク情報
DOI
https://doi.org/10.1007/s00441-020-03273-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32910242
ID情報
  • DOI : 10.1007/s00441-020-03273-y
  • PubMed ID : 32910242

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