2013年4月
A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine-Cytosine Base Pair Discriminates a Single-Nucleotide Difference in RNA
CHEMISTRY-A EUROPEAN JOURNAL
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- 巻
- 19
- 号
- 16
- 開始ページ
- 5034
- 終了ページ
- 5040
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/chem.201204183
- 出版者・発行元
- WILEY-V C H VERLAG GMBH
Selective discrimination of a single-nucleotide difference in single-stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)-derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine-containing DNA or RNA, effectively discriminates a single-nucleotide difference in a closely related group of sequences of single-stranded DNA and/or RNA. The pseudocomplementary PNA heteroduplex is easily converted to a fluorescent probe that distinctively detects a member of highly homologous let-7 microRNAs.
- リンク情報
- ID情報
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- DOI : 10.1002/chem.201204183
- ISSN : 0947-6539
- PubMed ID : 23494894
- Web of Science ID : WOS:000317399600013