2017年3月
Construction of a library of structurally diverse ribonucleopeptides with catalytic groups
BIOORGANIC & MEDICINAL CHEMISTRY
- ,
- ,
- ,
- 巻
- 25
- 号
- 6
- 開始ページ
- 1881
- 終了ページ
- 1888
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2017.02.007
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
Functional screening of structurally diverse libraries consisting of proteins or nucleic acids is an effective method to obtain receptors or aptamers with unique molecular recognition characteristics. However, further modification of these selected receptors to exert a newly desired function is still a challenging task. We have constructed a library of structurally diverse ribonucleopeptides (RNPs) that are modified with a catalytic group, in which the catalytic group aligns with various orientations against the ATP binding pocket of RNA subunit. As a proof-of-principle, the screening of the constructed RNP library for the catalytic reaction of ester hydrolysis was successfully carried out. The size of both the substrate-binding RNA library and the catalytic group modified peptide library are independently expandable, and thus, the size of RNPs library could be enlarged by a combination of these two subunits. We anticipate that the library of functionalized and structurally diverse RNPs would be expanded for various other catalytic reactions. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.bmc.2017.02.007
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28223024
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000396798900019&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/28223024
- URL
- http://orcid.org/0000-0001-9564-6805
- ID情報
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- DOI : 10.1016/j.bmc.2017.02.007
- ISSN : 0968-0896
- eISSN : 1464-3391
- ORCIDのPut Code : 34763358
- PubMed ID : 28223024
- Web of Science ID : WOS:000396798900019