2017年12月
Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae
Bioresource Technology
- 巻
- 245
- 号
- Pt B
- 開始ページ
- 1400
- 終了ページ
- 1406
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.biortech.2017.05.190
- 出版者・発行元
- ELSEVIER SCI LTD
This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/ mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, beta-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.biortech.2017.05.190
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28624243
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000413038300013&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-85020647423&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-7590-5320
- ID情報
-
- DOI : 10.1016/j.biortech.2017.05.190
- ISSN : 0960-8524
- eISSN : 1873-2976
- ORCIDのPut Code : 39500246
- PubMed ID : 28624243
- SCOPUS ID : 85020647423
- Web of Science ID : WOS:000413038300013