2013年
Theranostic protein targeting ErbB2 for bioluminescence imaging and therapy for cancer.
PloS one
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- 巻
- 8
- 号
- 9
- 開始ページ
- e75288
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1371/journal.pone.0075288
A combination of molecular-targeted cancer imaging and therapy is an emerging strategy to improve cancer diagnosis and minimize the side effects of conventional treatments. Here, we generated a recombinant protein, EC1-GLuc-p53C, by fusing EC1 peptide, an artificial ligand of ErbB2, with Gaussia luciferase (GLuc) and a p53-activating peptide, p53C. EC1-GLuc-p53C was expressed and purified from E. coli BL21. In vitro experiments showed that EC1-GLuc-p53c was stable in luminescent activity and selectively targeted ErbB2-overexpressing BT474 cells for bioluminescence imaging. Moreover, the internalized EC1-GLuc-p53C in BT474 cells exerted its function to reactivate p53 and significantly inhibited cellular proliferation. In tumor-bearing mice, the ErbB2-targeted bioluminescence imaging and therapeutic effect of EC1-GLuc-p53C were also observed specifically in BT474 tumors but not in MCF7 tumors, which does not overexpress ErbB2. Thus, the present study demonstrates EC1-GLuc-p53C to be an effective theranostic reagent targeting ErbB2 for bioluminescence imaging and cancer therapy.
- リンク情報
- ID情報
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- DOI : 10.1371/journal.pone.0075288
- PubMed ID : 24069396
- PubMed Central 記事ID : PMC3775930