Jul, 2008
Cell type dependent endocytic internalization of ErbB2 with an artificial peptide ligand that binds to ErbB2
CELL BIOLOGY INTERNATIONAL
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- Volume
- 32
- Number
- 7
- First page
- 814
- Last page
- 826
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.cellbi.2008.03.012
- Publisher
- ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
ErbB2, which is a member of the epidermal growth factor (erbB) receptor family, is frequently overexpressed in breast and ovarian cancers. Antibody and small molecule anti-tyrosine kinase inhibitors have been developed for targeted therapies for cancers overexpressing erbB2. Internalization and downregulation of erbB2, which is induced by a ligand, may be important for efficacious therapeutic effects. However, ligand-dependent erbB2 internalization has not been well characterized. Here we investigated the internalization of erbB2 in SKBr3 and SKOv3 cells, both overexpressing erbB2, using an EC-1 peptide fused to eGFP (EC-eGFP), which specifically binds to erbB2. ErbB2 was internalized in SKOv3 cells when the cells were treated with EC-eGFP. The accumulation of endosomal erbB2 was EC-eGFP dependent, which colocalized with transferrin implying endocytosis via clathrin-coated pits. In contrast, internalization of erbB2 was not observed in SKBr3 cells. As a result, two different mechanisms, which are cell type dependent for the internalization of erbB2, are proposed. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.cellbi.2008.03.012
- ISSN : 1065-6995
- Web of Science ID : WOS:000260671400014