Jul, 1998
Immunological characterization of alpha antigen of Mycobacterium kansasii: B-cell epitope mapping
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
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- Volume
- 48
- Number
- 1
- First page
- 73
- Last page
- 78
- Language
- English
- Publishing type
- Research paper (scientific journal)
- Publisher
- BLACKWELL SCIENCE LTD
Mapping of B-cell epitopes on alpha antigen of Mycobacterium kansasii (K-alpha) was carried out by using recombinant truncated K-alpha fusion peptides. We observed that two immunodominant B-cell epitopes (amino acids 222-268 and 267-306) and one minor epitope (amino acid 249-286) were located in the C-terminal region of K-alpha. The other three minor B-cell epitopes were mapped in N-terminal (amino acids 80-98 and 99-166) and central (amino acid 174-203) regions of K-alpha. All defined epitopes were common to Mycobacterium tuberculosis and M. kansasii. Besides these common epitopes, a region in K-alpha (amino acid 290-319) revealed different reactivities between antibodies against K-alpha and alpha antigen of M. tuberculosis. These findings may provide a basis for development of serodiagnosis that can distinguish between M. kansasii and M. tuberculosis infections.
- Link information
- ID information
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- ISSN : 0300-9475
- Pubmed ID : 9714413
- Web of Science ID : WOS:000074955500010