Feb, 1994
CYTOTOXIC T-LYMPHOCYTE RESPONSE IN MICE INDUCED BY A RECOMBINANT BCG VACCINATION WHICH PRODUCES AN EXTRACELLULAR ALPHA ANTIGEN THAT FUSED WITH THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE IMMUNODOMINANT DOMAIN IN THE V3 LOOP
VACCINE
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- Volume
- 12
- Number
- 2
- First page
- 153
- Last page
- 158
- Language
- English
- Publishing type
- Research paper (scientific journal)
- Publisher
- BUTTERWORTH-HEINEMANN LTD
The host immune response of cell-mediated immunity, particularly that of cytotoxic T lymphocytes (CTLs), is a major immune defence mechanism which may provide resistance to a human immunodeficiency virus type 1 (HIV-1) spread leading to acquired immune deficiency syndrome (AIDS). To prevent the accompanying activity of HIV-1 proteins responsible for the loss of helper T-lymphocyte function, it is crucial to develop a live attenuated recombinant vaccine expressing only T- or both T- and B-cell epitopes. Here, we examined the expression of the HIV-1 Env protein V3 region (15 amino acids from Arg(315) to Lys(329)) in Mycobacterium bovis BCG as a fused form with an extracellular alpha antigen of Mycobacterium kansasii. Balb/c mice inoculated with this recombinant BCG (rBCG), rapidly induced V3 peptide-specific CTLs. Target cell lysis was restricted to the murine class I major histocompatibility complex, H-2(d). A similar CTL response was also elicited after Balb/c mice were immunized with the same rBCG even when pre-inoculated with non-recombinant BCG. Thus, the rapid induction of HIV-1-specific CTLs indicates that this vaccine may be a therapeutic approach to preventing progression to AIDS.
- Link information
- ID information
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- ISSN : 0264-410X
- Pubmed ID : 8147098
- Web of Science ID : WOS:A1994MP25300010