論文

査読有り 国際誌
2018年1月15日

Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells.

Stem cells and development
  • Sayuri Hamano
  • ,
  • Atsushi Tomokiyo
  • ,
  • Daigaku Hasegawa
  • ,
  • Shinichiro Yoshida
  • ,
  • Hideki Sugii
  • ,
  • Hiromi Mitarai
  • ,
  • Shoko Fujino
  • ,
  • Naohisa Wada
  • ,
  • Hidefumi Maeda

27
2
開始ページ
100
終了ページ
111
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/scd.2017.0077
出版者・発行元
MARY ANN LIEBERT, INC

The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDL-related markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration.

リンク情報
DOI
https://doi.org/10.1089/scd.2017.0077
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29160151
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000418565200001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/scd.2017.0077
  • ISSN : 1547-3287
  • eISSN : 1557-8534
  • PubMed ID : 29160151
  • Web of Science ID : WOS:000418565200001

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