論文

査読有り
2017年8月

Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters

SCIENTIFIC REPORTS
  • Eiki Yuda
  • ,
  • Naoyuki Tanaka
  • ,
  • Takashi Fujishiro
  • ,
  • Nao Yokoyama
  • ,
  • Kei Hirabayashi
  • ,
  • Keiichi Fukuyama
  • ,
  • Kei Wada
  • ,
  • Yasuhiro Takahashi

7
1
開始ページ
9387
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-017-09846-2
出版者・発行元
NATURE PUBLISHING GROUP

Biogenesis of iron-sulfur (Fe-S) clusters is an indispensable process in living cells. In Escherichia coli, the SUF biosynthetic system consists of six proteins among which SufB, SufC and SufD form the SufBCD complex, which serves as a scaffold for the assembly of nascent Fe-S cluster. Despite recent progress in biochemical and structural studies, little is known about the specific regions providing the scaffold. Here we present a systematic mutational analysis of SufB and SufD and map their critical residues in two distinct regions. One region is located on the N-terminal side of the beta-helix core domain of SufB, where biochemical studies revealed that Cys254 of SufB (SufB(C254)) is essential for sulfur-transfer from SufE. Another functional region resides at an interface between SufB and SufD, where three residues (SufB(C405), SufB(E434), and SufD(H360)) appear to comprise the site for de novo cluster formation. Furthermore, we demonstrate a plausible tunnel in the beta-helix core domain of SufB through which the sulfur species may be transferred from SufB(C254) to SufB(C405). In contrast, a canonical Fe-S cluster binding motif (CxxCxxxC) of SufB is dispensable. These findings provide new insights into the mechanism of Fe-S cluster assembly by the SufBCD complex.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1038/s41598-017-09846-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28839209
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000408441600111&DestApp=WOS_CPL