論文

査読有り
2017年5月

Comparison among Japanese isolates of Pseudomonas savastanoi pv. savastanoi, causal agent of olive knot disease

JOURNAL OF GENERAL PLANT PATHOLOGY
  • Mizue Tsuji
  • ,
  • Kouki Ohta
  • ,
  • Kouta Tanaka
  • ,
  • Yuichi Takikawa

83
3
開始ページ
152
終了ページ
161
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10327-017-0710-2
出版者・発行元
SPRINGER JAPAN KK

Olive knot disease in Japan was first reported in Shizuoka Prefecture in 2014, and the causal agent was identified as Pseudomonas savastanoi pv. savastanoi. Subsequently, olive trees having knots were also found in Aichi and Kanagawa Prefectures in 2015, and the isolates from knots were also suspected to be P. savastanoi pv. savastanoi through preliminary examinations. Therefore, the Aichi and Kanagawa isolates were identified through comparison of isolates from three prefectures. Phylogenic analysis based on 16S rDNA and housekeeping genes (gyrB, rpoD, gltA and gap1) revealed that the isolates belonged to the same cluster as the pathotype strain, ICMP4352(PT). The iaaM, H and L genes, which are involved in promotion of symptoms, and the ina gene coding the ice nucleation protein, were detected by PCR from all the isolates. In rep-PCR (ERIC and REP) analyses, the isolates yielded DNA fragment-banding patterns that were nearly identical to that of ICMP4352(PT), but slight variations in banding patterns were observed among them. In a pathogenicity test, the isolates formed distinct knots on olive and pink jasmine. Phenotypic properties of the isolates were almost identical to those of ICMP4352(PT), with the exception of d-sorbitol utilization. Consequently, Aichi and Kanagawa isolates from olive were identified as P. savastanoi pv. savastanoi, and several genetic diversities in terms of rep-PCR were found in the Japanese population of P. savastanoi pv. savastanoi, indicating their heterogeneity.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1007/s10327-017-0710-2
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000399697500005&DestApp=WOS_CPL