論文

査読有り
2012年8月

Novel In Situ Pretreatment Method for Significantly Enhancing the Signal In MALDI-TOF MS of Formalin-Fixed Paraffin-Embedded Tissue Sections

PLOS ONE
  • Yu Kakimoto
  • ,
  • Tatsuaki Tsuruyama
  • ,
  • Takushi Yamamoto
  • ,
  • Masaru Furuta
  • ,
  • Hirokazu Kotani
  • ,
  • Munetaka Ozeki
  • ,
  • Akihiko Yoshizawa
  • ,
  • Hironori Haga
  • ,
  • Keiji Tamaki

7
8
開始ページ
e41607
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0041607
出版者・発行元
PUBLIC LIBRARY SCIENCE

The application of matrix-assisted laser desorption/ionization (MALDI)-based mass spectrometry (MS) to the proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue presents significant technical challenges. In situ enzymatic digestion is frequently used to unlock formalin-fixed tissues for analysis, but the results are often unsatisfactory. Here, we report a new, simplified in situ pretreatment method for preparing tissue sections for MS that involves heating with vapor containing acetonitrile in a small airtight pressurized space. The utility of the novel method is shown using FFPE tissue of human colon carcinoma. The number and intensity of MALDI peaks obtained from analysis of pretreated tissue was significantly higher than control tissue not subjected to pretreatment. A prominent peak (m/z 850) apparently specific to cancerous tissue was identified as a fragment of histone H2A in FFPE tissue pretreated using our method. This highly sensitive treatment may enable MALDI-MS analysis of archived pathological FFPE samples, thus leading to the identification of new biomarkers.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0041607
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201502869950587119
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22899997
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307380900012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0041607
  • ISSN : 1932-6203
  • J-Global ID : 201502869950587119
  • PubMed ID : 22899997
  • Web of Science ID : WOS:000307380900012

エクスポート
BibTeX RIS