論文

査読有り 国際誌
2022年6月22日

Ladder observation of bovine serum albumin by high resolution agarose native gel electrophoresis.

International journal of biological macromolecules
  • Yui Tomioka
  • ,
  • Masataka Nakagawa
  • ,
  • Chiaki Sakuma
  • ,
  • Satoru Nagatoishi
  • ,
  • Kouhei Tsumoto
  • ,
  • Tsutomu Arakawa
  • ,
  • Teruo Akuta

215
開始ページ
512
終了ページ
520
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ijbiomac.2022.06.118

A commercially available bovine serum albumin (BSA) was examined by agarose native gel electrophoresis using two different agarose sources, UltraPure and MetaPhor agarose. While UltraPure agarose up to 5 % showed no clear separation of BSA oligomers, MetaPhor agarose clearly demonstrated oligomer bands above 4 %, indicating that the latter agarose has greater molecular sieving effects and is hence characterized to have high resolution for size differences, as probed by a greater slope of Ferguson plot. Physical properties are different between two agaroses. In general, UltraPure agarose has physical strength, while MetaPhor agarose is considerably fragile, but MetaPhor agarose solution is less viscous so that even 10 % gel can be made. Cause of oligomers was shown to be not associated with inter-chain disulfide bonds, but is due to association of native or native-like molecules.

リンク情報
DOI
https://doi.org/10.1016/j.ijbiomac.2022.06.118
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35752339
ID情報
  • DOI : 10.1016/j.ijbiomac.2022.06.118
  • PubMed ID : 35752339

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