2009年6月
Functional evidence for Eme1 as a marker of cisplatin resistance
INTERNATIONAL JOURNAL OF CANCER
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- 巻
- 124
- 号
- 12
- 開始ページ
- 2997
- 終了ページ
- 3001
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/ijc.24268
- 出版者・発行元
- WILEY-LISS
The ability to predict cisplatin sensitivity in tumors has been expected to greatly improve the outcome of cancer therapy because the drug is frequently used in a variety of tumors. Although ERCC1 and other repair proteins have be gated as markers of cisplatin resistance, reliable markers are still needed. Here, we demonstrate that Eme1 levels can predict cisplatin sensitivity more accurately than ERCC1 or Rad51 levels in a variety of human cancer cell lines. Eme1 forms a heterodimeric protein complex with Mus81 and functions as a structure-specific endonuclease. Haploinsufficiency of Eme1 led to hypersensitivity to cisplatin in the colon cancer cell line HCT116. On the basis of this finding, we examined the relationships between levels of proteins involved in the repair of interstrand cross-links and cisplatin sensitivity in human tumor cell lines with a variety of origins. Although ERCC1, Rad51 and Mus81 levels correlated with sensitivity to some extent, the clearest correlation was observed with Eme1. Tumors with low Eme1 levels were more sensitive to the drug than tumors with high levels. This suggests that the measurement of Eme1 in tumors may be more informative for cisplatin-based chemotherapy
- リンク情報
- ID情報
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- DOI : 10.1002/ijc.24268
- ISSN : 0020-7136
- PubMed ID : 19267403
- Web of Science ID : WOS:000265997500033