論文

査読有り
2017年2月

Pulmonary platelet accumulation induced by catecholamines: Its involvement in lipopolysaccharide-induced anaphylaxis-like shock

INTERNATIONAL IMMUNOPHARMACOLOGY
  • Zhiqian Yu
  • ,
  • Hiroko Saito
  • ,
  • Hirotada Otsuka
  • ,
  • Yosuke Shikama
  • ,
  • Hiromi Funayama
  • ,
  • Mai Sakai
  • ,
  • Shigeo Murai
  • ,
  • Masanori Nakamura
  • ,
  • Takashi Yokochi
  • ,
  • Haruhiko Takada
  • ,
  • Shunji Sugawara
  • ,
  • Yasuo Endo

43
開始ページ
40
終了ページ
52
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.intimp.2016.11.034
出版者・発行元
ELSEVIER SCIENCE BV

Intravenously injected lipopolysaccharides (LPS) rapidly induce pulmonary platelet accumulation (PPA) and anaphylaxis-like shock (ALS) in mice. Macrophages reportedly release catecholamines rapidly upon stimulation with LPS. Here, we examined the involvement of macrophage-derived catecholamines in LPS-induced PPA and ALS. A catecholamine or Klebsiella O3 (KO3) LPS was intravenously injected into mice, with 5-hydroxytryptamine in the lung being measured as a platelet marker. The tested catecholamines induced PPA, leading to shock Their minimum shock-inducing doses were at the nmol/kg level. The effects of epinephrine and norepinephrine were inhibited by prazosin (alpha 1 antagonist) and by yohimbine (alpha 2 antagonist), while dopamine's were inhibited only by prazosin. Use of synthetic adrenergic alpha 1- and/or alpha 2-agonists, platelet- or macrophage-depleted mice, a complement C5 inhibitor and C5-deficient mice revealed that (a) alpha 2-receptor-mediated PPA and shock depend on both macrophages and complements, while alpha 1-receptor-mediated PPA and shock depend on neither macrophages nor complements, (b) the PPA and ALS induced by KO3-LPS depend on alpha 1- and alpha 2-receptors, macrophages, and complements, and (c) KO3-LPS-induced PPA is preceded by catecholamines decreasing in serum. Together, these results suggest the following. (i) Catecholamines may stimulate macrophages and release complement C5 via alpha 2-receptors. (ii) Macrophage-derived catecholamines may mediate LPS-induced PPA and ALS. (iii) Moderate PPA may serve as a defense mechanism to remove excess catecholamines from the circulation by promoting their rapid uptake, thus preventing excessive systemic effects. (iv) The present findings might provide an insight into possible future pharmacological strategies against such diseases as shock and acute respiratory distress syndrome. (C) 2016 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1016/j.intimp.2016.11.034
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27939824
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000393242800006&DestApp=WOS_CPL